class BisearchEnzimHu::FpcrSubmitter
Public Class Methods
new(url=URL)
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# File lib/bisearch_enzim_hu/fpcr_submitter.rb, line 8 def initialize(url=URL) @url = url end
Public Instance Methods
run(hash, index)
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# File lib/bisearch_enzim_hu/fpcr_submitter.rb, line 13 def run(hash, index) fp = hash[:fp] || hash[:line_a][:seq] # forward primer rp = hash[:rp] || hash[:line_b][:seq] # reverse primer fpcr = "#{fp}!#{rp}" db = "Homo sapiens" params = { "bis" => "on", # bisulfite "db" => db, # database "fp" => fp, # forward primer "fpcr" => fpcr, # "fpcr_but.x" => "35", "fpcr_but.y" => "6", "mm" => "0000000011111111", # mismatches "npcrres" => "100", # PCR product to show "nprimerres" => "100", # Primer matches to show "prg" => "cgi/fpcr.cgi", "rp" => rp # reverse primer } uri = URI(@url) puts "---> starting FPCR" t = Time.now page = Net::HTTP.post_form(uri, params) puts "---> FPCR completed (time: #{Time.now-t}s)" # puts "--- save result to file: fpcr_result_#{index}.html" # File.open("fpcr_result_#{index}.html", 'w') {|f| f.write page.body } result_page = BisearchEnzimHu::L2ResultPage.new(page.body) result_page.parse # return an hash end