RPA {chipPCR} | R Documentation |
Real-time amplification plot of an Recombinase Polymerase Amplification (RPA) by Lutz et al.(2009) in a centrifugal microfluidic foil cartridge.
data("RPA")
A data frame with 184 observations on the following 2 variables.
Reaction.Time
Reaction Time [min]
RFU
Relative fluorescence units [RFU]
The data were digitized as described by Poisot (2011). The image for data extraction was taken from Figure 3b by Lutz et al.(2009). The amplification curve present the results of a cross-contamination test of a foil disk for a sample containing 2000 copies of the mecA gene. For further experimental and technical details refer to Lutz et al.(2009).
The original data (open access under CC BY-NC-ND license) were taken from Figure 3b by Lutz et al.(2009).
Poisot, T. (2011). The digitize Package: Extracting Numerical Data from Scatterplots. The R Journal 3, 25–26.
Lutz, S., Weber, P., Focke, M., Faltin, B., Roth, G., Piepenburg, O., Armes, N., Mark, D., Zengerle, R., and von Stetten, F. (2009). Isothermal Polymerase Amplification in a Centrifugal Microfluidic Foil Cartridge. Procedia Chemistry 1, 529–531.
data(RPA)
plot(RPA, main = "RPA by Lutz et al.(2009)", xlab = "Reaction Time [min]",
ylab = "Relative fluorescence units [RFU]", type = "l")
abline(h = 5, lty = 2, col = "grey")