IgG_inhibition {guescini} | R Documentation |
This data set is for a set of quantitative real-time PCR runs that were
performed in the presence of an optimal amplification reaction mix added with
serial dilutions of IgG (0.0, 0.25, 0.50, 1.0, and 2.0 \mu g/ml
) thus acting as
the inhibitory agent. There are two replicates for each concentration of IgG.
The concentration of the amplicon ND1/ND2 is 41,700,000 copies.
Please read the Methods section of Guescini et al. (2008) for more details.
A tibble with 400 rows and 10 variables:
plate
Plate identifier.
well
Well identifier. Values are always NA
(not available). This
variable is kept nevertheless to be coherent with other data sets from other
similar R data packages.
dye
The type of dye used. In this data set the values are always
"SYBR"
, meaning SYBR Green I master mix (Roche).
target
Target identifier: the amplicon used, "MT_ND1"
.
sample_type
Sample type (all curves are standards, i.e. "std"
).
replicate
Replicate identifier: 1 thru 3.
IgG_conc
IgG concentration in \mu g/ml
.
copies
Standard copy number.
cycle
PCR cycle.
fluor
Raw fluorescence values.
IgG_inhibition