s1 {boggy} | R Documentation |
The qPCR data set named s1 comprises a six-point, ten-fold dilution series, replicated in duplicates targeting an amplicon designed with a random sequence that minimizes secondary structure and off-target primer binding. Please read the Quantitative PCR data section, qPCR assays subsection of Boggy et al. (2010) for more details.
A tibble with 480 rows and 10 variables:
plate
Plate identifier. For this data set it is always missing
(NA
).
well
Well identifier.
dye
The type of dye used. In this data set the values are always
"SYTO 13"
, meaning 2 mM of the dsDNA dye SYTO-13 (Invitrogen, Carlsbad, CA).
target
Target identifier: the amplicon used, a random sequence
dubbed "S1"
by the original authors.
sample_type
Sample type (all curves are standards, i.e. "std"
).
replicate
Replicate identifier: either 1 or 2.
copies
Standard copy number.
dilution
Dilution factor. Higher number means greater dilution.
cycle
PCR cycle.
fluor
Raw fluorescence values.
doi:10.1371/journal.pone.0012355
s1