normalize_RFU {quicR}R Documentation

Normalize Fluorescence

Description

Normalizes the real-time RT-QuIC data against the background fluorescence of a defined cycle. All cycles are divided by the fluorescent value of the defined cycle.

Usage

normalize_RFU(df, bg_cycle = 4)

Arguments

df

A dataframe made from get_real.

bg_cycle

The cycle used for background fluorescence

Value

A dataframe containing real-time normalized fluorescence values.

Examples

# This test takes >5 sec

file <- system.file(
  "extdata/input_files",
  file = "test.xlsx",
  package = "quicR"
)
df_ <- get_real(file)[[1]]

# Export the tables in the first sheet of the file.
dic <- quicR::organize_tables(file)

# Apply the column names.
colnames(df_) <- cbind("Time", convert_tables(dic)$`Sample IDs` |> t())

# Normalize the raw data against the background reading.
normalize_RFU(df_)




[Package quicR version 1.0.2 Index]