reclassify_droplets {ddpcr} | R Documentation |
Analysis step: Reclassify droplets
Description
After classifying droplets into clusters in each well individually, it may
be useful to attempt to reclassify droplets in wells where the gates are
not very clearly defined. This function uses information taken from wells
with a high negative frquency (where the gate is easily defined) to adjust
the gates in the other wells.
See the README for
more information about the algorithm used.
Usage
reclassify_droplets(plate)
Details
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze
function) rather than being called
directly.
Note
This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.
See Also
analyze
reclassify_droplets_single
mark_clusters