remove_outliers {ddpcr} | R Documentation |
Analysis Step: Remove outlier droplets
Description
Identify droplets that have an abnormally high fluorescence intensity as
outliers. Any such droplets will be assigned to the OUTLIER cluster.
See the README for
more information about the algorithm used to find outlier droplets.
Usage
remove_outliers(plate)
Arguments
plate |
A ddPCR plate. |
Details
This function is recommended to be run as part of an analysis pipeline (ie.
within the analyze
function) rather than being called
directly.
Value
A ddPCR plate with outlier droplets marked as outliers. The plate's
metadata will have a new variable drops_outlier
which will count the
number of outlier droplets in each well.
Note
This is an S3 generic, which means that different ddPCR plate types can implement this function differently. See the README for more information on how to implement custom ddPCR plate types.