get_filled_drops {ddpcr} | R Documentation |
Get filled droplets in PNPP experiment
Description
In a PNPP experiment, the rain droplets are the non-empty drops that don't have a
high enough intensity in the positive dimension to be considered as filled
with high quality sample DNA. Only droplets considered as filled are
candidates for the negative
and positive
clusters.
get_filled_drops
returns the droplets that are considered filled.
Usage
get_filled_drops(plate, well_id, border)
Arguments
plate |
A ddPCR plate. |
well_id |
Get border of filled droplets for this well. |
border |
(Optional) The filled droplets border, as calculated by
|
Value
Dataframe with all filled droplets in the given well.
See Also
pnpp_experiment
positive_dim
get_filled_drops
Examples
file <- system.file("sample_data", "small", "analyzed_pnpp.rds", package = "ddpcr")
plate <- load_plate(file)
get_filled_drops(plate, "A05")
get_filled_drops(plate, "A05", get_filled_border(plate, "A05"))
[Package ddpcr version 1.15.2 Index]