PlateVision: Automated qPCR Analysis and Visual Quality Control

Description

A straightforward tool designed to directly pipe raw qPCR machine outputs into downstream analyses using the comparative Ct (Delta-Delta Ct) method described by Livak and Schmittgen (2001) doi:10.1006/meth.2001.1262. The package streamlines the workflow from Excel export to publication-ready plots. It integrates unique visual quality control by reconstructing 96-well plate heatmaps, allowing users to instantly detect pipetting errors, edge effects, and outliers. Key features include automated error propagation, laboratory master mix calculations, and generation of bar charts and volcano plots.

Author(s)

Maintainer: Shubham Love shubhamlove2101@gmail.com