R: Calculate Master Mix Reagents

calculate_reagents {PlateVision}

R Documentation

Calculate Master Mix Reagents

Description

Generates a recipe for your qPCR Master Mix based on sample count.

Usage

calculate_reagents(
  n_samples,
  n_genes,
  replicates = 3,
  rxn_volume = 20,
  dead_volume_pct = 10
)

Arguments

n_samples

Number of biological samples.

n_genes

Number of genes (targets + reference).

replicates

Number of technical replicates (usually 3).

rxn_volume

Total volume per well (e.g., 20 uL).

dead_volume_pct

Percentage of extra mix to prepare for pipetting error (default 10%).

Value

A data frame containing the mix recipe.

Examples

# Plan for 12 samples, 2 genes, standard 20uL reaction
calculate_reagents(n_samples = 12, n_genes = 2)

# Adjust for a 10uL reaction volume and 15% dead volume
calculate_reagents(12, 2, rxn_volume = 10, dead_volume_pct = 15)

[Package PlateVision version 0.1.0 Index]