smoke-package {smoke} | R Documentation |
Small Molecule Octet/BLI Kinetics Experiment
Description
Bio-Layer Interferometry (BLI) is a technology to determine the binding kinetics between biomolecules. BLI signals are small and noisy when small molecules are investigated as ligands (analytes). We develop this package to process and analyze the BLI data acquired on Octet Red96 from Fortebio more accurately. Sun Q., Li X., et al (2020) <doi:10.1038/s41467-019-14238-3>. In this new version, we organize the BLI experiment data and analysis methods into a S4 class with self-explaining structure.
Details
The DESCRIPTION file:
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'Smoke' is an object-based package to analyze kinetics data from BioLayer Interferometry (BLI). The example dataset was published in Nature Communication 2020, 11: 339.
Author(s)
Qingan Sun, Xiaojun Li, James C Sacchettini
Maintainer: Qingan Sun <quinsun@gmail.com>
References
Sun Q, Li X, Perez LM, Shi W, Zhang Y, Sacchettini JC (2020). “The molecular basis of pyrazinamide activity on Mycobacterium tuberculosis PanD.” Nature Communications, 11(339).
Examples
bli1 <- alignLoad(bli, 180, 780)
bli2 <- doubleBlank(bli1)
bli3 <- baseline(bli2, 1080, 1260)
bli4 <- estimate(bli3)
bli5 <- fitKinetics(bli4)
plotTraces(bli2)
plotKinetics(bli5)
plotResiduals(bli5)